Chelation of lead by dithizone with UV-Vis detection was the first truly sensitive method for the determination of lead. It was added from German munitions measurements. It requires excruciating attention to cleanliness. Can you meet those standards?

The peak of interest overlaps with the blank peak. You will need to deconvolute it or baseline subtract the peaks. The exercise with deconvolutions can be found in Part I of the “Geek Page”.

Learning how to create a calibration curve WITH confidence limits should help you decide if or if not you meet the standards of yesterday. The instructions for confidence limits can be found in Part I of the “Geek Page”.

For a little more understanding of absorbance instrumentation is included an exercise in altering “equipment” within the “instrument”: gratings and angle of incidence. These can be found under Part V of the “Geek Page”